Data were analyzed us-ing 1-way ANOVA models with pair-wise group differences in mean cholesterol level assessed using Tukey post hoc tests. Enter the email address you signed up with and we'll email you a reset link. BODIPY‐Cholesterol: A New Tool to Visualize Sterol ... - DeepDyve that binds to a specific receptor on the surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis. Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). BODIPY-cholesterol can be reliably used to monitor cholesterol efflux ... a cholesterol-Bodipy (Bdp-Chol) derivative (Fig. Results are based on five (endogenous cholesterol) six (BODIPY-cholesterol) independent samples and presented as the mean percent BODIPY-cholesterol or endogenous cholesterol remaining ± SEM. ) synthesized a cholesterol-Bodipy (Bdp-Chol) derivative (Fig. TopFluor® Cholesterol - Avanti Polar Lipids A 08 angle corresponds to BODIPY-cholesterol standing upright in a manner where BODIPY is in the membrane center. Editorial, see p 542 . Briefly, following dilution to 250 × 10 6 sperm/mL with TALP devoid of BSA and bicarbonate, spermatozoa were labelled with 1 µM BODIPY-cholesterol (1 mM stock solution . BODIPY-cholesterol in WT cells was 1.43 ± 0.07 pmol/μg protein and in M12 cells was 1.90 ± 0.17 pmol/μg protein (mean ± SEM, n = 4). Bodipy-cholesterol has been shown to behave similarly to native cholesterol in cultured vertebrate cells . CholEsteryl BODIPY 542/563 C11 | C54H77BF2N2O3 | CID 135563652 - structure, chemical names, physical and chemical properties, classification, patents, literature . Apolipoprotein E gene polymorphism influences aggressive behavior in ... BODIPY-cholesterol complements intrinsically fluorescent cholesterol analogues. Upon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. (Invitrogen) . a cholesterol assay demonstrating that β-catenin knockdown decreases the cholesterol concentration. MMPC Immunohistochemistry BODIPY bodipy-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent bodipy group at carbon 24. Cholesteryl Ester Transfer Protein Variants Have Differential Stability ... Fetal bovine serum (FBS) and DMEM were obtained from Gibco BRL (Life Technologies, Paisley, Scotland), if not stated otherwise.

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